What are mcf7 breast cancer cells?
1 MCF-7 human adenocarcinoma cells. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27] …
What does triple negative breast cancer mean?
What Is Triple-Negative Breast Cancer? Triple-negative breast cancer is a kind of breast cancer that does not have any of the receptors that are commonly found in breast cancer. Think of cancer cells as a house. The front door may have three kinds of locks, called receptors— One is for the female hormone estrogen.
Is MCF7 triple negative?
MCF7 and MDA-MB-231 cells represent a striking example in that they are both invasive ductal/breast carcinoma cells, yet they have many phenotypic/genotypic differences: MCF7 are hormone dependent (both estrogen and progesterone receptor positive—ER and PR), while MDA-MB-231 are triple negative.
What is the doubling time for MDA-MB-231?
about 38h
Hi Nairrita, the doubling time of MDA-MB-231 cells is about 38h according to ATCC. I usually start with the density of 7,500 cells/cm2 in 96-well plate for 3 days MTS assay.
Are SKBR3 cells metastatic?
3), indicating that SKBR3/c-Jun derived cells formed tumors, and metastasized to the liver.
What are the effects of leptin on MDA-mb-361 cells?
In MDA-MB-361 cells, Ob-Rb and VEGF increased at 24 and 48 h; whereas PI3K, Stat3, PCNA and insulin levels increased in leptin-treated MDA-MB-361 cells after 48 h. Bcl-2 and IGF-IRalpha were decreased at 24 h and a dose-dependent increase at 48 h was noted.
What is the role of mitochondrial estrogen receptors in breast cancer?
Functional estrogen receptors in the mitochondria of breast cancer cells. Mol Biol Cell. 2006;17:2125–37. 29. Razandi M, Pedram A, Jordan VC, Fuqua S, Levin ER. Tamoxifen regulates cell fate through mitochondrial estrogen receptor beta in breast cancer.
Which MDA-MB-453 cells have low levels of ER-beta?
While T47DCo (a T47D variant cell line), BT474, MDA-MB-231, MDA-MB-453, MDA-MB-468 and MCF-7 express low levels of ER-beta, other cell lines including the T47D-Y (a T47D variant cell line), MDA-MB-435, BT-549, and SKBr-3 cells express undetectable levels of ER-beta.
How do selective estrogen receptor modulators work?
Selective estrogen receptor modulators (SERM), such as tamoxifen and raloxifene bind to ERs, antagonize the effect of estrogen on specific target genes along with a partial agonistic effect.