What is shRNA plasmid?
Short hairpin RNA (shRNA) sequences are usually encoded in a DNA vector that can be introduced into cells via plasmid transfection or viral transduction. shRNA molecules can be divided into two main categories based on their designs: simple stem-loop and microRNA-adapted shRNA.
What is shRNA lentivirus?
Small hairpin RNA (shRNA) and lentiviral vector RNA interference has been used as a research tool to control the expression of specific genes in numerous experimental organisms and has potential as a therapeutic strategy to reduce the expression of problem genes[1,2].
What is the role of shRNA?
A short hairpin RNA or small hairpin RNA (shRNA/Hairpin Vector) is an artificial RNA molecule with a tight hairpin turn that can be used to silence target gene expression via RNA interference (RNAi).
What is shRNA transfection?
Transfection is the process of introducing purified nucleic acids [deoxyribonucleic acid (DNA) or ribonucleic acid (RNA)] into cultured eukaryotic cells via viral or nonviral methods.
What is shRNA used for?
How long does shRNA take to work?
Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
How long does shRNA knockdown last?
5–7 days
Gene silencing resulting from siRNA can be assessed as early as 24 hours post-transfection. The effect most often will last from 5–7 days. However, the duration and level of knockdown are dependent on the cell type and concentration of siRNA. Transfections may be repeated to maintain silencing.
Is shRNA stable?
Altogen Labs offers development of inducible RNAi stable cell lines expressing small hairpin RNA (shRNA) constructs that target genes of interest. These stable cell lines expressing shRNA constructs are important expression research tools for studying gene function in mammalian cells.
What are the different features of shRNA vectors?
These shRNA vectors contain different features, such as different fluorescent protein markers and/or mammalian selection markers. Contact Us| Register or Login| Cart0| ×Close
What is the smartvector shRNA platform?
The SMARTvector microRNA-adapted shRNA platform shows considerably higher mature strand activity and lower passenger stand activity than simple hairpin shRNAs targeting the same gene. The result of the optimized microRNA-adapted scaffold is higher, more specific function while reducing the risk of off-target effects from the passenger strand.
Which shRNA cloning vectors are best for gene function study?
Short Hairpin (shRNA) Cloning Vectors | OriGene shRNA mediated gene knockdown is still a popular gene function study tool. OriGene has 10 shRNA cloning vectors, including retroviral, lentiviral and AAV shRNA vectors. These shRNA vectors contain different features, such as different fluorescent protein markers and/or mammalian selection markers.
How are shRNA sequences transcribed?
Traditional short hairpin RNA (shRNA) sequences are transcribed in the nucleus from a vector containing a Pol III promoter. The shRNA, containing the sense and antisense sequences from a target gene connected by a loop, is transported from the nucleus into the cytoplasm where the enzyme Dicer processes it into small/short interfering RNAs (siRNAs).