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What is intravital fluorescence microscopy?

What is intravital fluorescence microscopy?

Intravital microscopy is a powerful imaging tool for visualizing various biological processes, simultaneously. This allows for in vivo study of several critical cell types that can be difficult to fully understand when present in cell cultures, such as neurons or tumor cells.

What is multiphoton fluorescence microscopy?

Multiphoton fluorescence microscopy is a powerful research tool that combines the advanced optical techniques of laser scanning microscopy with long wavelength multiphoton fluorescence excitation to capture high-resolution, three-dimensional images of specimens tagged with highly specific fluorophores.

What does intravital?

Definition of intravital 1 : performed upon or found in a living subject. 2 : having or utilizing the property of staining cells within a living body — compare supravital.

What does intranasal use only mean?

: lying within or administered by way of the nasal structures.

Why do lasers produce multiphoton processes?

Compared with linear excitation, the first practical advantage of multiphoton excitation is that a laser source with longer emission wavelength can be used, which is often less difficult to make, because a nonlinear frequency conversion stage is not needed.

What is multi photon absorption process?

Multiphoton absorption is the term used to describe a process in which an atom or molecule makes a single transition between two of its allowed energy levels by absorbing the energy from more than a single photon.

Which drugs may be used intranasally?

There are many classes of medications that may be used intranasally, many of which are applicable to the pre-hospital and emergency setting. These medications include antiepileptics, opiate analgesics and opiate antagonists, sedatives, topical anesthetics, glucagon for hypoglycemia, and agents for epistaxis control.

What is two-photon luminescence?

Two-photon fluorescence microscopy allows three-dimensional imaging of biological specimens in vivo. Compared with confocal microscopy, it offers the advantages of deeper tissue penetration and less photodamage but has the disadvantage of slightly lower resolution. Nature of Two-photon Absorption.

Why is two-photon absorption a third order process?

Two-photon absorption (TPA) is a third order nonlinear optical phenomenon in which a molecule absorbs two photons at the same time. The transition energy for this process is equal to the sum of the energies of the two photons absorbed.