What does CD45 stand for?
CD45 (lymphocyte common antigen) is a receptor-linked protein tyrosine phosphatase that is expressed on all leucocytes, and which plays a crucial role in the function of these cells.
What is CD45 RA or ro?
The CD45RA/RO assay is used in the evaluation of immunodeficiency. CD45RA and CD45RO are isoforms of the CD45 antigen that are typically expressed on T Cells in identifiable patterns using flow cytometry.
What is normal range for CD45?
Data were recorded as the mean fluorescence intensity (MFI) for signal and noise. Signal-to–noise (S/N) ratio was calculated for CD45 expressed lymphocytes. In healthy adults, the MFI and S/N ratio of CD45 expression for the whole lymphocyte population (n = 14) was 82.2 and 62.24, respectively.
Is CD45 a cell surface marker?
As cell surface immunophenotype marker, CD45 is of great value in differentiation of lymphoproliferative diseases subtypes.
Is CD45 on all immune cells?
CD45 is a major transmembrane glycoprotein expressed on all nucleated hematopoietic cells. Eight isoforms of CD45 are distributed through the immune system according to cell type and degree of cellular differentiation.
What is anti CD45?
A monoclonal antibody directed against the receptor-like leukocyte cell surface glycoprotein CD45 with leukocyte-depleting activity.
What is CD45 dim?
The CD45-dim area is often the gating strategy used to identify and phenotype blasts in multiple tubes and is useful and convenient in most samples. However, one should consider the presence of non blasts” as possible heterogeneous populations in this CD45-dim area in order to avoid erroneous results.
What does low CD45 mean?
CD45 is a transmembrane tyrosine phosphatase involved in T cell receptor signaling and T cell development in the thymus. A deficiency of CD45 leads to a marked impairment in T cell development. 5. This diagnosis should be suspected in patients with a T-B+NK+ SCID phenotype with absent CD45 surface expression.
What does CD45 negative mean?
negative cell populations). CD45-negative non-hematopoietic cell populations may be seen in a variety of samples received in clinical flow cytometry laboratories for the workup of leukemia/lymphoma especially those exhibiting morphologic features of round blue-cell tumors that mimic lymphoma.
What does CD45-negative mean?
What does high CD45 mean?
Predominant presence of CD45− PCs in the bone marrow indicates late stage of the disease and appears to portend a less favorable prognosis. Further studies are needed in order to characterize all the functional differences between these population and the factors associated with the progression.
What cells stain for CD45?
The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. This antibody is specific for mouse CD45 and suitable for immunohistochemical staining of Zinc-fixed and formalin fixed paraffin sections as well as acetone-fixed, frozen sections.
What does CD45 bind?
T cells require the protein tyrosine phosphatase CD45 to detect and respond to antigen because it activates the Src family kinase Lck, which phosphorylates the T cell antigen receptor (TCR) complex. CD45 activates Lck by opposing the negative regulatory kinase Csk.
What cells are CD45 +?
CD45 is a type I transmembrane protein that is present in various isoforms on all differentiated hematopoietic cells (except erythrocytes and plasma cells). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.
What is CD45 lymphoma?
CD45 is a glycoprotein expressed in all lymphohemopoietic cells. Its expression increases during B-lymphocyte ontogeny. Few data are available about CD45 expression in the various types of low-grade B-cell non-Hodgkin’s lymphomas (NHL).
What type of cells express CD45?
CD45 is a transmembrane protein tyrosine phosphatase located on most haematopoietic cells. It has several isoforms, and haematopoietic cells express one or more of the isoforms—CD45RO, CD45RA and CD45RB. CD45 immunoreactivity is recognised to be highly specific for non–Hodgkin’s lymphomas.