What color is Alexa Fluor 647?
far-red
Invitrogen Alexa Fluor 647 dye is a bright, far-red–fluorescent dye with excitation ideally suited for the 594 nm or 633 nm laser lines.
Is Alexa Fluor 647 the same as APC?
Due to nearly identical excitation and emission properties, APC and Alexa Fluor® 647 cannot be used simultaneously. APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications.
Is Alexa Fluor 647 a tandem?
Due to nearly identical excitation and emission properties, but different spillover characteristics, APC and Alexa Fluor 647 cannot be used simultaneously.
Is Alexa 647 Cy5?
For stable signal generation, Alexa Fluor 647 dye is generally brighter than Cy5 dye in protein and nucleic acid conjugates. Allophycocyanin (APC) is another bright fluorophore that is spectrally equivalent to Cy5 dye and Alexa Fluor 647 dye, but it is used almost exclusively in flow cytometry.
Which fluorophores are brightest?
It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
Which is brighter PE or APC?
It is also because APC is red-shifted compared to PE. Thus, the light source used to maximally excite APC will contain less energy than the one used to maximally excite PE. Compared to other red fluorophores such as Cy5, however, APC is still significantly brighter and an excellent choice for use in flow cytometry.
What is the difference between fluorophore and fluorochrome?
Yes, fluorophore and fluorochrome refer to the same thing. Fluorophores, or fluorochromes, are fluorescent chemical compounds that are capable of absorbing light from a laser and re-emitting the light within a range of wavelengths upon excitation.
Does FITC bleed into PE?
Why do we need compensation? where FITC bleeds into the PE channel and PE bleeds back into FITC. To correct for spectral overlap during multicolor flow cytometry experiments, color compensation must be performed. The goal of color compensation is to correctly quantify each dye with which a particular cell is labeled.
Is FITC same as 488?
High-performance alternatives to fluorescein The Invitrogen Molecular Probes Alexa Fluor 488 dye—with spectral properties and quantum yield nearly identical to those of fluorescein isothiocyanate (FITC)—produces brighter, more photostable conjugates.
Can you use APC and PE Cy5 together?
For example, PE-Cy™5 is very bright with regard to stain index (Table 1), but it has considerable spillover (ie, adds background) into the APC detector. While these two fluorochromes can be used together, the resolution sensitivity in APC will be reduced compared to, for example, a combination of PerCP-Cy5. 5 and APC.
Can I use PerCP and PerCP Cy5 5 together?
Anyways, the two dyes cannot be used simultaneously as there is overlap in their emission spectra. And so you got the spectral overlap. As these two dyes are tandem dyes and in both of them the reciepient dye is cy5, they could not be used simultanously.
Which fluorescent dye can be used for red fluorescence?
Rhodamine. The spirolactam structure of rhodamine is nonfluorescent and colorless and exhibits high selectivity for metal ions; the rhodamine ring in its open form has strong fluorescence.
Is GFP and FITC the same?
Green Fluorescent Protein (GFP) Antibody (FITC) is a Rabbit Polyclonal antibody conjugated to FITC against GFP.