What is rhodamine B isothiocyanate?
Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat.
What is the role of rhodamine B?
Rhodamine B /ˈroʊdəmiːn/ is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers.
What does rhodamine stain in cells?
Rhodamine auramine stain is used for the detection of mycobacteria directly from clinical specimens. The dye binds with the mycolic acids and fluoresces under ultraviolet light. Acid fast organisms (mycobacteria) will appear yellow or orange under ultraviolet light.
Is rhodamine B an acid or base?
Rhodamine B is a xanthene dye whose optical properties depend of several factors, such as solvent, concentration and pH value [2]. The carboxyl group participates of typical acid-base balance, wherein the acidic and basic forms are strongly luminescent and colored [2].
Is Rhodamine B an azo dye?
Rhodamine dye. dyes, half the number belong to the class of Azo dyes[1][2]. Technology, P.O. Box 652, Cape Town 8000, South Africa.
What are forward and side scatter?
Forward versus side scatter (FSC vs SSC) gating is commonly used to identify cells of interest based on size and granularity (complexity). It is often suggested that forward scatter indicates cell size whereas side scatter relates to the complexity or granularity of the cell.
What is rhodamine B dye?
Description. Rhodamine B is an organic chloride salt having N-[9-(2-carboxyphenyl)-6-(diethylamino)-3H-xanthen-3-ylidene]-N-ethylethanaminium as the counterion. An amphoteric dye commonly used as a fluorochrome. It has a role as a fluorochrome, a fluorescent probe and a histological dye.
Is rhodamine B water soluble?
Rhodamine B base is also used as a laser dye, tunable around 610 nm with the fluorescence yield being temperature dependent. Solubility : Soluble in ethanol (1 mg/ml), methanol, chloroform, and DMF. Insoluble in water.
Why is Rhodamine B banned?
Rhodamine is a banned dye as per PFA act (1954), by Government of India, because the same dye has found to be carcinogenic in human. It is harmful to humans because of its carcinogenicity, reproductive and developmental toxicity, neurotoxicity, and acute toxicity.
How do you dissolve Rhodamine B?
Dissolve 0.1 g of sample of rhodamine B in 100 mL of water. Not more than a faint trace of turbidity or insoluble matter should remain. Dissolve 0.1 g of sample in alcohol. Not more than a faint trace of turbidity or insoluble matter should remain.
Is Rhodamine B water soluble?
What is Rhodamine B isothiocyanate?
1S/C29H29N3O3S.ClH/c1-5-31 (6-2)20-10-13-23-26 (16-20)35-27-17-21 (32 (7-3)8-4)11-14-24 (27)28 (23)22-12-9-19 (30-18-36)15-25 (22)29 (33)34;/h9-17H,5-8H2,1-4H3;1H Rhodamine B isothiocyanate (RBITC) is a fluorescent dye. It acts as an anterograde and retrograde marker of retinal neurons in the adult rat.
Is Rhodamine B adsorbed from ethanolic solution?
(c) Rhodamine B physically adsorbed from ethanolic solutions and subsequent removal of solvent by evaporation. These results show that the positive charge density on the nitrogen atom is smaller for dyed samples when compared with the adsorbed ones.
Is Rhodamine B safe to eat?
In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label. Cases of economically motivated adulteration, where it has been illegally used to impart a red color to chili powder, have come to the attention of food safety regulators.
What is the fluorescence of rhodamine B at room temperature?
The fluorescence yield is temperature dependent; the compound is fluxional in that its excitability is in thermal equilibrium at room temperature. In California, rhodamine B is suspected to be carcinogenic and thus products containing it must contain a warning on its label.