What type of breast cancer is MCF-7?
4.8. MCF-7 is a human breast cancer cell line with estrogen, progesterone and glucocorticoid receptors [26]. It is derived from the pleural effusion of a 69-year-old Caucasian metastatic breast cancer (adenocarcinoma) in 1970 by Dr Soule of the Michigan Cancer Foundation, Detroit, MI [27].
What does MCF-7 stand for?
MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old White woman. MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers.
What does proliferation mean in cancer?
Cell proliferation is how quickly a cancer cell copies its DNA and divides into 2 cells. If the cancer cells are dividing more rapidly, it means the cancer is faster growing or more aggressive.
What are MCF-7 cells used for?
MCF-7 cells are useful for in vitro breast cancer studies as a result of the cell line retaining several ideal characteristics particular to the mammary epithelium. These include the ability for MCF-7 cells to process estrogen in the form of estradiol via estrogen receptors in the cell cytoplasm.
How do MCF-7 cells grow?
The common medium for culturing MCF-7 cells is Eagle’s MEM (EMEM), supplemented with 10% FBS and 1% penicillin/streptomycin, non essential amino acids (0.1 mM), insulin (10 ug/mL) and sodium pyruvate (1 mM). Add 10 nM estrogen to media for a 3-4 x increase in cell population growth.
What does proliferation mean in medical terms?
An increase in the number of cells as a result of cell growth and cell division.
How fast do MCF-7 cells grow?
MCF-7 human mammary carcinoma cells were inoculated into 150-sq cm flasks at 3 X 10(5) cells/flask, and after a lag period of about 48 hr, these cells grew exponentially for 5 days with a mean population doubling time of about 24 hr.
What is an example of proliferation?
Proliferation is a rapid increase in numbers or rapid growth. When a country is quickly building up more weapons including nuclear weapons, this is an example of nuclear proliferation. If you start out with one rabbit and within a month you end up with five, this is an example of proliferation of rabbits.
What does proliferation mean in biology?
What causes proliferation?
Cell proliferation occurs by combining cell growth with regular “G1-S-M-G2” cell cycles to produce many diploid cell progeny. In single-celled organisms, cell proliferation is largely responsive to the availability of nutrients in the environment (or laboratory growth medium).
What is a proliferation in medical terms?