What is an example of a restriction enzyme?
SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end. Other restriction enzymes, like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other.
What is HindIII restriction enzyme?
HindIII (pronounced “Hin D Three”) is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the DNA palindromic sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.
How do restriction enzymes recognize DNA sequences?
When a restriction endonuclease recognizes a sequence, it snips through the DNA molecule by catalyzing the hydrolysis (splitting of a chemical bond by addition of a water molecule) of the bond between adjacent nucleotides.
Do exonuclease produce sticky ends?
Exonucleases, unlike endonucleases, do not have a lag period as they cleave the sequences from the ends, resulting in sticky ends.
What is EcoRI and HindIII?
Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer.
Why do restriction enzymes recognize palindromic sequences?
DNA is double stranded, so it has ‘two sides’ to which the enzyme can bind. A palindromic sequence is the same backwards and forwards on both sides (see image below). This means that the enzyme recognizes the sequence no matter from which side the enzyme approaches the DNA.
Is Type III an endonuclease?
Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites.
Who discovered Hind 2?
Hamilton Smith
(Science: enzyme molecular biology) first type II restriction endonuclease identified, by Hamilton Smith in 1970. Isolated from haemophilus influenzae, it cleaves the sequence GTPyPuAC between the unspecified pyrimidine and purine generating blunt ends.