What are anti tubulin antibodies?
Product Description. Rabbit anti Porcine tubulin antibody recognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
What is the molecular weight of beta tubulin?
approximately 50 kiloDaltons
Beta-Tubulin Molecular Weight Each beta tubulin and alpha tubulin has a molecular weight of approximately 50 kiloDaltons.
What is alpha and beta tubulin?
Definition. Alpha tubulin refers to one of the two protein components of a microtubule attached to GTP while beta tubulin is the second protein component of a microtubule. Thus, this is the fundamental difference between alpha and beta tubulin.
What is the molecular weight of alpha tubulin?
50 kDa
Alpha tubulin molecular weight: 50 kDa.
What is the role of a tubulin gel?
Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division.
What is alpha tubulin antibody?
The α-Tubulin Antibody detects endogenous levels of total α-tubulin protein, and does not cross-react with recombinant β-tubulin.
How big is a tubulin dimer?
roughly 50 kDa
Tubulin subunits that make up microtubules have comparable dimensions. To be more precise, tubulin dimers made up of alpha and beta tubulin subunits (each of mass roughly 50 kDa) form protofilaments with a periodicity of 8 nm.
What is alpha tubulin?
The TUBA1A gene provides instructions for making a protein called alpha-tubulin (α-tubulin). This protein is part of the tubulin family of proteins that form and organize structures called microtubules. Microtubules are rigid, hollow fibers that make up the cell’s structural framework (the cytoskeleton).
What is tubulin polymerization?
Tubulin dimers polymerize to form microtubules, which generally consist of 13 linear protofilaments assembled around a hollow core (Figure 11.37). The protofilaments, which are composed of head-to-tail arrays of tubulin dimers, are arranged in parallel.
What is gamma tubulin?
γ-tubulin belongs to the tubulin family protein that is conserved in eukaryotic cells. This molecule localizes at the centrosome and required for microtubule nucleation from the centrosome. gtbl+ gene was isolated by its homology to Aspergillus nidulans γ-tubulin gene (23).
Where is alpha tubulin found in a cell?
Overview. Alpha and beta tubulins are globular proteins and exist included in five main forms/subunits as well as delta, gamma, and epsilon. As building blocks of various structures, tubulins belong to a group of proteins found in the cell cytoplasm in high amounts.
What size are microtubules?
about 25 nanometers
Microtubules are the largest type of filament, with a diameter of about 25 nanometers (nm), and they are composed of a protein called tubulin.
Does alpha tubulin bind to GTP?
Each α- and β-tubulin molecule binds one molecule of GTP (Figures 1B,C).